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一.產口名稱：Human MMP-9 ELISA Kit

二.Human MMP-9 ELISA Kit簡介：

Matrix metalloproteinases (MMPs) are a family of zinc-dependent  endopeptidases that degrade extracellular matrix proteins. MMPs have been linked with a wide array of biological activities and play important roles during organ development and pathological processes. Collectively MMPs are key enzymes for the metabolism of extracellular matrix proteins, including fibrillar and non-fibrillar collagens, fibronectin, laminin and basement membrane or interstitial stroma glycoproteins. Under physiological
conditions MMPs are involved in extracellular degradation and breakdown of matrix proteins during normal tissue remodelling processes such as wound healing, pregnancy, and angiogenesis. Human MMP-9 is a 92 kDa glycoprotein that plays a significant role in matrix remodeling, enzyme modulation, and cytokine/growth factor activation. MMP-9 is also known as gelatinase B based on its ability to degrade gelatin.
 

The RayBiotech  Human MMP-9 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human MMP-9 pro and active
forms in serum, plasma (Collect plasma using heparin as an anticoagulant. EDTA and Citrate are not recommended), cell culture supernatants and urine.This assay employs an antibody specific for human MMP-9 coated on a 96- well plate. Standards and samples are pipetted into the wells and MMP-9
present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human MMP-9 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of MMP-9 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

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